A Calorimetric Study on the Interaction of Zinc and Cadmium Ions with Jack Bean Urease
✍ Scribed by G. Rezaei Behbehani; A. A. Saboury; F. Sabbaghy
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 90 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0256-7660
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✦ Synopsis
Abstract
A thermodynamic study on the interaction of Jack bean urease, JBU, with Zn^2+^ and Cd^2+^ ions was studied by isothermal titration calorimetry (ITC) at 290, 300 and 310 K in 30 mmol/L Tris buffer solution, pH 7.0. The heats of JBU+Zn^2+^ and JBU+ ^2+^ interactions are reported and analyzed in terms of the extended solvation theory. It was indicated that there is a set of 12 identical and non‐interacting binding sites for Zn^2+^ and Cd^2+^ ions. The interactions of Zn^2+^ and Cd^2+^ ions with JBU are exothermic and both enthalpy and entropy driven. The association equilibrium constants for JBU+Zn^2+^ complexes are 4118.20, 3354.70 and 2790.62 L·mol^−1^ at 290, 300 and 310 K respectively. The association equilibrium constants for JBU+Cd^2+^ interactions are 2831.6 and 2386.28 L· ^−1^ at 300 and 310 K, respectively.