A Bowman-Birk trypsin inhibitor with antiproliferative activity from Hokkaido large black soybeans
✍ Scribed by Vincent S. M. Ho; T. B. NG
- Book ID
- 105360739
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 123 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1075-2617
- DOI
- 10.1002/psc.922
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✦ Synopsis
Abstract
A trypsin inhibitor, with an N‐terminal sequence highly homologous to those of 8‐kDa Bowman‐Birk trypsin inhibitors, was isolated from the seeds of Hokkaido large black soybeans. The trypsin inhibitor was unadsorbed on SP‐Sepharose but adsorbed on DEAE‐cellulose and Mono Q. It inhibited proliferation in breast cancer (MCF‐7) cells and hepatoma (Hep G2) cells with an IC~50~ of 35 and 140 µM, respectively. The trypsin inhibitory activity of the inhibitor was completely preserved after exposure to temperatures up to 100 °C for 30 min and to the pH range 2–13 for the same duration. The trypsin inhibitor inhibited HIV‐1 reverse transcriptase with an IC~50~ of 38 µM, but was devoid of antifungal activity toward Fusarium oxysporum and Mycosphaerella arachidicola. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.