A bovine whole-genome radiation hybrid panel and outline map
✍ Scribed by John L. Williams; André Eggen; Luca Ferretti; Christine J. Farr; Mathieu Gautier; Giuseppe Amati; Glynn Ball; Tiziana Caramorr; Ricky Critcher; Sandro Costa; Patrick Hextall; David Hills; Aurore Jeulin; Susanna L. Kiguwa; Olivia Ross; Angela L. Smith; Katiana Saunier; Barbara Urquhart; Dave Waddington
- Publisher
- Springer-Verlag
- Year
- 2002
- Tongue
- English
- Weight
- 254 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0938-8990
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## Summary We have constructed a radiation hybrid (RH) map of the porcine genome using an RH panel generated by an irradiation dose of 5000‐rad (Sus scrofa radiation hybrid map, SSRH map). Normal porcine aortic endothelial cells were irradiated and fused with a thymidine kinase‐deficient mouse cell
Twelve bovine ribosomal protein genes, for which sequence data had been acquired from complementary deoxyribonucleic acid (cDNA) clones isolated from a cattle skin cDNA library, were mapped. As ribosomal protein genes are a group of highly conserved house keeping genes, specific primers were designe