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A biosynthetic thiolase in complex with a reaction intermediate: the crystal structure provides new insights into the catalytic mechanism

✍ Scribed by Yorgo Modis; Rik K Wierenga


Book ID
114328824
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
292 KB
Volume
7
Category
Article
ISSN
0969-2126

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## Abstract Thymidine‐3′‐monophosphate (3′‐TMP) is a competitive inhibitor analogue of the 3′‐CMP and 3′‐UMP natural product inhibitors of bovine pancreatic ribonuclease A (RNase A). Isothermal titration calorimetry experiments show that 3′‐TMP binds the enzyme with a dissociation constant (__K__~d