A bioluminescent assay for 12-cr-hydroxy bile acids was developed using enzymes coimmobilized onto Sepharose 4B. The immobilized enzymes used were a bacterial 12-a-hydroxysteroid dehydrogenase, bacterial luciferase, and NADPHPMN oxidoreductase or bacterial diaphorase. The assay was specific for 12+h
A bioluminescence assay for total 3α-hydroxy bile acids in serum using immobilized enzymes
✍ Scribed by Juergen Schoelmerich; Gerard P. van Berge Henegouwen; Alan F. Hofmann; Marlene DeLuca
- Book ID
- 115824832
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 854 KB
- Volume
- 137
- Category
- Article
- ISSN
- 0009-8981
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