A Better Start in Life: : Intracytoplasmic Morphologically Selected Sperm Injection (IMSI)
✍ Scribed by Sandrine Batard; Christian Lainé
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2008
- Weight
- 294 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1439-4243
No coin nor oath required. For personal study only.
✦ Synopsis
The success of in vitro fertilisation techniques such as intracytoplasmic sperm injection (ICSI) depends critically on selection of the most healthy sperm for injection into the oocyte. Intracytoplasmic morphologically selected sperm injection (IMSI) is a high magnification light microscopy imaging method that is being used increasingly to select sperm for ICSI. IMSI has been shown to increase pregnancy rates and reduce abortion rates compared to routine ICSI-IVF. This technical note describes IMSI using a Nikon inverted microscope equipped with Normarski DIC optics and digital imaging system to achieve magnifications in excess of 6000x.