๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A Batch Elution Procedure for Assaying Adenylate Cyclase

โœ Scribed by S.P.J. Brooks; M.J. Mancebo; C.P. Holden; K.B. Storey


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
286 KB
Volume
210
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


A new assay for measuring adenyl cyclase
โœ John L. Humes; Marcia Rounbehler; Frederick A. Kuehl Jr. ๐Ÿ“‚ Article ๐Ÿ“… 1969 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 439 KB

who provided us with some details of this salt procedure, unpublished at that time.

A batch procedure for the assay of cycli
โœ Clark Bublitz ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 296 KB

A procedure for the assay of cyclic nucleotide phosphodiesterase is described in which labeled cyclic nucleotide is separated from labeled nucleoside by the batchwise addition of ethanolic slurries of Dowex 2 fluoride. Under the conditions described there is no detectable adsorption of nucleoside by

A modified assay of adenylate cyclase us
โœ Simon van Heyningen; Avril Thom; Walter Ward ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 147 KB

548) has been modified by using [3H]ATP to produce [3H]cyclic AMP and monitoring the recovery with [14C]cyclic AMP. The modified assay remains sensitive and accurate, but avoids the inconvenience and expense of using 32p.

Adenylate cyclase assay with [ฮฑ-32P]ATP
โœ Raymond Counis; Sylvie Mongongu ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 414 KB

In the assay of adenylate cyclase using [&\*P]ATP as the substrate and alumina chromatography as the separating procedure for labeled nucleotides, blank levels are dependent on the quality of the labeled ATP and also on that of the alumina. In order to lower the blanks by eliminating the radioactive