✦ LIBER ✦
A 59Co NMR relaxation probe of macromolecule anionic binding sites
✍ Scribed by T. Raj; C.R. Bennett; R.G. Bryant
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 299 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Wo NMR linewidth measurements are reported which show that hexacyanocobaltate-(III) ion may be used as a sensitive probe of protein interactions with anions in aqueous solutions. Applications are demonstrated to bovine serum albumin where the probe ion binding is monitored as a function of pH and is displaced from the protein sites by hexacyanoferrate(II1) ion. The general utility of complex metal ions is suggested as a generally useful approach to the analysis of ion-macromolecule interactions.