A [4,5-3H]lysine:[14C]lysine dual-label method to measure lysine hydroxylation in collagen

A new method for determining the extent

A new method for determining the extent of proline hydroxylation by measuring changes in the ratio of [4-3H]:[14C]proline in collagenase digests

✍ Mario Chojkier; Beverly Peterkofsky; John Bateman 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 689 KB

A new method has been developed to determine the extent of proline hydroxylation in collagen. This method relies on the measurement of changes in the ratio of [3H]:['4C]proline in bacterial collagenase digests, resulting from specific loss of tritium from the 4-trans position of the proline during h