A 43-base-pair complementary DNA sequence homology and triplet repeat motif among putative polymeric immunoglobulin receptor messenger RNAs in regenerating rat liver
✍ Scribed by K. S. Koch; R. G. Fletcher; M. P. Grond; H. L. Leffert
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 436 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
is interested in the control of hepatic proliferation. Recently, we described a cyclin messenger RNA (mRNA) and p32/p34CDK2'cd'2 histone H1-kinase cascade during liver regeneration after partial hepatectomy (1). This procedure also induces patterned reexpression of fetal and neonatal genes and large numbers of proto-oncogenes (2).
While screening a rat poly(A) + complementary DNA (cDNA) library from 24-hr regenerating liver (constructed by X.P. Lu in this laboratory) at slightly reduced stringency (l), with a 1,245-bp human cDNA probe encoding full-length cyclin D1 mRNA (31, we isolated three clones containing cDNA inserts of approximately 208 to 342 bp from a Stratagene Lambda Uni-ZapXR plasmid expression vector (Stratagene, La Jolla, CA) (see Fig. 1 and "Note Added in Proof '1. Thus