A 30-kDa protein is disulfide linked to IgM on normal and neoplastic murine B lymphocytes
✍ Scribed by Dieter Haustein; Dietmar Von Der Ahe
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 367 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Four cloned continuously cultured mouse B lymphoma cells of the lines WEHI-279, BCLl and 38C-13 were used to study the surface presentation of IgM. Cells of all lines expose, in addition to F&, various disulfide-linked subunits as already shown for normal B lymphocytes (Koch, N. and Haustein, D., Mol. Zmmunol. 1982.19: 477). In contrast to normal B cells, B lymphoma cells also carry IgM structures of higher molecular weight than p2L on their surface. Furthermore, B lymphoma cells of all investigated lines expose a p2L2 structure to which a 30-kDa protein is disulfidelinked. This 30-kDa protein could also be identified on normal mouse B lymphocytes when more drastic conditions were employed. p.& disulfide linked to this 30-kDa protein is the main IgM structure on normal and neoplastic B cells whereas only small amounts of kZLz (unlinked to this protein) can be detected.