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A 1H-NMR method for determining temperature in cell culture perfusion systems

✍ Scribed by N. W. Lutz; A. C. Kuesel; W. E. Hull

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
560 KB
Volume
29
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

This report describes a noninvasive ^1^H‐NMR method for measuring absolute temperatures (Β±0.2Β°C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature‐independent shift of one of the components of the biological medium, e.g., pyruvate, acetate, or lactate. The effects of flow on temperature can be monitored and appropriate adjustments of the temperature controller can be made.

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