A 16 kb small single-copy region separates the plastid DNA inverted repeat of the unicellular red algaCyanidium caldarium: physical mapping of the IR-flanking regions and nucleotide sequences of thepsbD-psbC, rps16, 5S rRNA andrpl21genes
✍ Scribed by Udo Maid; Klaus Zetsche
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 849 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
The four inverted repeat (IR) flanking regions of the Cyanidium caldarium plastid DNA were cloned. Southern blotting, transcript and sequence analyses of the border regions revealed the psbD-psbC operon and the rps16 gene within the large single-copy region upstream of the 16S rDNA gene and the rpl21 gene downstream of the 5S rDNA within the 16 kb small single-copy region. The size of the IR is ca. 5 kb. The nucleotide sequences of the psbD-psbC, rps16, rp121 and 5S rRNA genes with the corresponding alignments and physical maps of the regions are presented. Northern analysis revealed a less complex psbD-psbC transcription pattern than has been found in higher plants. Comparisons to other red algal data point to structural diversity within red algal plastid DNA.