A 1 : 2 Copper(II)–Tripeptide Complex for DNA Binding and Cleavage Agent under Physiological Conditions
✍ Scribed by Pulimamidi R. Reddy; Pallerla Manjula; Tushar K. Chakraborty; Rajarshi Samanta
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 383 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1612-1872
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✦ Synopsis
Abstract
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A 1 : 2 copper–tripeptide complex, [Cu^II^(Boc‐His‐Gly‐His‐OMe)~2~]^2+^, was synthesized and structurally characterized. The absorption band at 577 nm suggests a square‐planar geometry around Cu^II^. The DNA‐binding and DNA‐cleavage properties of the Cu^II^ complex were investigated. The complex binds to calf thymus DNA (CT DNA) in an intercalative fashion and cleaves plasmid pUC‐19 DNA hydrolytically at micromolar concentrations under physiological conditions. The intrinsic binding constant (K~b~=1.2×10^2^ M^−1^) for the binding of Cu–tripeptide complex with DNA suggests that this complex is suitable for rapid diffusion on the pharmacological time scale.