99m-technetium labelling of a tumor associated murine monoclonal antibody for immunoscintigraphic studies in man

✍ Scribed by Dr. Spyridon C. Archimandritis; Julia Malamitsi; Elias Belkas; Alexandra D. Varvarigou; Kyriakos Cotsyfakis; Gregory Sivolapenko; John Zorzos; Demosthenes V. Skarlos; Charalambos Proukakis

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: French
• Weight: 513 KB
• Volume: 34
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580340404

✦ Synopsis

Abstract

The present study refers to the preparation of a 99m‐Technetium labelled murine monoclonal antibody for clinical application. The monoclonal antibody was incubated with a 20fold molar excess of 2‐iminothiolane. The free thiol groups created, were capable of binding reduced technetium. Labelling took place through an exchange reaction with 99m‐Technetium‐Glucoheptonate. The labelling conditions were studied extensively. Diverse chromatographic methods (ITLC, HPLC) were developed in order to study the radiochemical behaviour of the labelled antibody and evaluate the labelling efficiency and the in vitro stability. Results on the labelling technique and on the quality control methods are presented, as well as preliminary immunoscintigraphic data.