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Batch'digestion experiments were conducted to determine the effects of inoculum:substrate ratio and initial medium pH on methane production from glucose. A ratio between the chemical oxygen demands of the substrate and inoculum solids (SISR) was used to quantify the food to microorganisms ratio. Two periods of active methane production, separated by a period of inactivity, characterized the fermentation. The duration of inactivity was affected by both SISR and the initial medium pH. It decreased as SISR was decreased or when a higher initial pH was used. With a SISR of 4.8 and a pH of 7.2 or 7.0, the inactive period was eliminated, indicating satisfactory couplin methane yield at a s of the acid-and methane-forming phases. Ultimate ISR of 4.8 was higher than at a SISR OF 38.5.
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Observation of possibilities and problems was performed when trying to optimise growing conditions for high biomass production by irrigation and fertilisation in a clone test of poplar on sandy soil in the south of Sweden. One hundred and eight clones of pure Populus trichocarpa and hybrids between