𝔖 Bobbio Scriptorium
✦   LIBER   ✦

96-Well apparatus and method for use in enzyme-linked immunofiltration assay (ELIFA)

✍ Scribed by C R Clark; K K Hines; A K Mallia

Book ID
104646512
Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
369 KB
Volume
7
Category
Article
ISSN
0951-208X

No coin nor oath required. For personal study only.

✦ Synopsis

An apparatus and method are described for use in enzyme-linked immunofiltratlon assay (ELIFA). The method of filtering ligands, blockers, wash buffers, and substrates decreases a typical 30 min to 2 hr incubation step with ELISA to 5 minutes. The apparatus differs from a dot-blot apparatus in that soluble coloured product is transferred directly into a receiving 96-well microtiter plate for quantifying with an automatic plate reader. Model systems of streptavidin/biotin and antibody/antigen form detectable complexes in the apparatus.

πŸ“œ SIMILAR VOLUMES

In Situ Deprotection: A Method for Coval
In Situ Deprotection: A Method for Covalent Immobilization of Peptides with Well-Defined Orientation for Use in Solid Phase Immunoassays such as Enzyme-Linked Immunosorbent Assay
✍ Klaus Gregorius; Michael Theisen πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 116 KB

The orientation of an immobilized antigen is important for recognition by, e.g., an antibody. When noncovalent passive adsorption is used for immobilization, the number of ways that the antigen can attach to the surface is numerous and control of how the antigen orientates on the surface is limited.