9,10-Anthraquinone Modified Glassy Carbon Electrode and Its Application for Hemoglobin Determination

✍ Scribed by Zhiwei Zhu; Nan-Qiang Li

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 80 KB
• Volume: 10
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/(sici)1521-4109(199807)10:9<643::aid-elan643>3.0.co;2-6

✦ Synopsis

The electrochemical behavior of a glassy carbon electrode modified with 9,10-anthraquinone has been investigated in the absence and presence of hemoglobin. The stability of the modified electrode is satisfactory. Cyclic voltammogram of 9,10-anthraquinone modified electrode in 0.15 mol L ¹1 pH 7.0 phosphate buffer exhibits a redox wave at ¹0.557 V (vs. SCE). Upon adding hemoglobin in solution, both of the reduction and oxidation currents decrease with increasing peak separation. It was found that the decrease of differential pulse voltammetric current was proportional to the concentration of hemoglobin over the range from 5.0 × 10 ¹8 to 3.0 × 10 ¹7 mol L ¹1 and from 3.0 × 10 ¹7 to 1.0 × 10 ¹6 mol L ¹1 (with correlation coefficients of 0.9971 and 0.9982, respectively). A satisfactory result was obtained for the determination of hemoglobin in clinical blood samples.