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67Ga-citrate distribution in solid hepatoma 22

✍ Scribed by F. Zayas; J. Oliva; A. Lage; J. W. Díaz; L. F. Alcorta; M. Warder; G. Lastra

Book ID
104682513
Publisher
Springer
Year
1984
Tongue
English
Weight
376 KB
Volume
9
Category
Article
ISSN
0340-6997

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✦ Synopsis

The intracellular distribution of 67Ga was studied in solid hepatoma 22 implanted in C3Ha/6 mice and in normal liver tissue from the same animals at different time intervals. The tissues were fractionated according to differential centrifugation principles, and subcellular fractions were isolated consecutively. The enzyme activities and the accumulation of 67Ga were determined in each fraction. The subcellular distribution of 67Ga in the tumor tissue was different compared with normal liver; in tumor it was found mainly in the nuclear fraction and this distribution was independent of time, but in normal liver the accumulation was mainly in the mitochondrial fraction, this was time-dependent and the maximal uptake was found 48 h after 67Ga administration.

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