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6-Oxy-(acetyl ethylenediamine) fluorescein, a novel fluorescent derivatization reagent for carboxylic acids and its application in HPLC

✍ Scribed by Xiao-Lan Du; Hong Wang; Ming Ma; Ying-Hua Deng; Hua-Shan Zhang

Book ID: 102926901
Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 693 KB
Volume: 31
Category: Article
ISSN: 1615-9306
DOI: 10.1002/jssc.200700456

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✦ Synopsis

Abstract

A novel fluorescent derivatization reagent for carboxylic acids, 6‐oxy‐(acetyl ethylenediamine) fluorescein (AEF), was well designed, synthesized, and applied to HPLC. The derivatization reaction with 12 fatty acids, including n‐valeric acid (C5), n‐hexanoic acid (C6), n‐heptanoic acid (C7), n‐octanoic acid (C8), n‐nonanoic acid (C9), n‐decanoic acid (C10), lauric acid (C12), myristic acid (C14), palmitic acid (C16), stearic acid (C18), oleic acid (C18:1), and linoleic acid (C18:2), was completed at 55°C within 40 min. The derivatives of fatty acids were separated on a C18 RP column and detected by fluorescence detection. The LODs attained were 0.4–1.2 nM (S/N of 3). It has been demonstrated that AEF is a prominent derivatization reagent for carboxylic acids which is suitable for HPLC.

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