𝔖 Bobbio Scriptorium
✦   LIBER   ✦

5,7,3′-trihydroxy-3,4′-dimethoxyflavone-induced cell death in human Leukemia cells is dependent on caspases and activates the MAPK pathway

✍ Scribed by Fernando Torres; José Quintana; Francisco Estévez

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
400 KB
Volume
49
Category
Article
ISSN
0899-1987

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Flavonoids are polyphenolic compounds which display a vast array of biological activities and are promising anticancer agents. In this study we investigated the effect of 5,7,3′‐trihydroxy‐3,4′‐dimethoxyflavone (THDF) on viability of nine human tumor cell lines and found that it was highly cytotoxic against leukemia cells. THDF induced G~2~–M phase cell‐cycle arrest and apoptosis through a caspase‐dependent mechanism involving cytochrome c release, processing of multiple caspases (caspase‐3, ‐6, ‐7, and ‐9) and cleavage of poly(ADP‐ribose) polymerase. Overexpression of the protective mitochondrial proteins Bcl‐2 and Bcl‐x~L~ conferred partial resistance to THDF‐induced apoptosis. This flavonoid induced the phosphorylation of members of the mitogen‐activated protein kinases (MAPKs) family and cell death was attenuated by inhibition of c‐jun N‐terminal kinases/stress‐activated protein kinases (JNK/SAPK) and of extracellular signal‐regulated kinases (ERK) 1/2. In the present study we report that THDF‐induced cell death is mediated by an intrinsic dependent apoptotic event involving mitochondria and MAPKs, and through a mechanism independent of the generation of reactive oxygen species. The results suggest that THDF could be useful in the development of novel anticancer agents. Mol. Carcinog. © 2010 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Betuletol 3-methyl ether induces G2-M ph
Betuletol 3-methyl ether induces G2-M phase arrest and activates the sphingomyelin and MAPK pathways in human leukemia cells
✍ Sara Rubio; José Quintana; José L. Eiroa; Jorge Triana; Francisco Estévez 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 365 KB

Betuletol 3-methyl ether (BME) is a natural phenylbenzo-gamma-pyrone that inhibits cell proliferation in human tumor cell lines and induces apoptotic cell death in HL-60 cells. Here we show that BME displays strong cytotoxic properties in several human leukemia cell lines (U937, K-562, THP-1, Jurkat

β2-microglobulin induces caspase-depende
β2-microglobulin induces caspase-dependent apoptosis in the CCRF-HSB-2 human leukemia cell line independently of the caspase-3, -8 and -9 pathways but through increased reactive oxygen species
✍ John Gordon; Ching-Huang Wu; Mojgan Rastegar; Ahmad R. Safa 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 426 KB

## Abstract Exogenous β~2~‐microglobulin (β~2~m) induces significant apoptosis in the CCRF‐HSB‐2 human lymphoblastic leukemia cell line as detected by DNA fragmentation, DAPI staining and annexin V binding assay. β~2~m treatment induced the release of cytochrome c and apoptosis‐inducing factor (AIF