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5286636 DNA cloning vectors with in vivo excisable plasmids

✍ Scribed by Frank, Daniel S.; Dunleavy, Katie; Nambaziira, Rashidah; Nayebare, Irene; Dreifuss, Bradley; Bisanzo, Mark


Book ID
121971594
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
63 KB
Volume
12
Category
Article
ISSN
0734-9750

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πŸ“œ SIMILAR VOLUMES


Molecular cloning with bifunctional plas
✍ Ostroff, G. R. ;PΓ¨ne, J. J. πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 793 KB

Cloning in Escherichia coli and Bacillus subtilis was carried out using the bifunctional plasmid pDH5060. B. subtilis chromosomal DNA and pDH5060 DNA were digested with either BamHI or SalI, then annealed, ligated, and transformed into E. coli SK2267. Transformants containing sequences ligated into

Molecular cloning with bifunctional plas
✍ Ostroff, G. R. ;PΓ¨ne, J. J. πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 692 KB

Recombinant plasmid DNA cloned in E. coli via the bifunctional vector pDH5060 suffered deletions when returned to B. subtilis. However, DNA preparations of identical chimeras containing homologous or heterologous sequences stably transformed B. subtilis at high efficiency when isolated from B. subti

Molecular cloning of complementary DNA:
✍ Anne Leriche; Daniel Christophe; Huguette Brocas; Gilbert Vassart πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 281 KB

A simple method that allows the rapid preparation of oligo dG-tailed plasmid vectors is presented. The procedure involves purification of the tailed molecules by hybridization to oligo dC-cellulose followed by a stepwise thermal elution. The resulting plasmid is virtually devoid of transformation ac