5286636 DNA cloning vectors with in vivo excisable plasmids
β Scribed by Frank, Daniel S.; Dunleavy, Katie; Nambaziira, Rashidah; Nayebare, Irene; Dreifuss, Bradley; Bisanzo, Mark
- Book ID
- 121971594
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 63 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0734-9750
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Recombinant plasmid DNA cloned in E. coli via the bifunctional vector pDH5060 suffered deletions when returned to B. subtilis. However, DNA preparations of identical chimeras containing homologous or heterologous sequences stably transformed B. subtilis at high efficiency when isolated from B. subti
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