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5281519 Simple, rapid and reliable method for detecting thalassemia

Book ID
122940605
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
68 KB
Volume
12
Category
Article
ISSN
0734-9750

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A rapid and simple electrophoretic metho
A rapid and simple electrophoretic method for the detection of mutations involving small insertion or deletion: application to ฮฒ-thalassemia
โœ Shi-Ping Cai; Barry Eng; Yuet Wai Kan; David H. K. Chui ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 581 KB

The 1.8-kb beta-globin gene fragments of DNAs from individuals heterozygous for nine different beta-thalassemia mutations involving 1, 2, 3, 4, or 25 basepair (bp) insertions or deletions were amplified by the polymerase chain reaction (PCR). The PCR products were subjected to electrophoresis on aqu