✍ Scribed by Matias, W. G. ;Creppy, E. E.
No coin nor oath required. For personal study only.
Okadaic acid OA , a toxin produced by dinoflagellates, accumulates in the hepatopancreas of mussels and causes diarrhetic shellfish poisoning. This toxin is a tumor promoter and a specific potent inhibitor of protein phosphatases 1 and 2A. The effect of OA on the relative amounts of the five ( nucleosides deoxycytosine, 5-methyldeoxycytosine, desoxythymidine, deoxyguanine, and deoxyade-) nine in the DNA of Vero cell cultures was determined by high performance liquid chromatography in
[ 3 ] parallel to DNA synthesis followed by H -thymidine incorporation. OA at a concentration of 7.5 ng / mL did not significantly inhibit DNA synthesis, but induced hypermethylation in Vero cell DNA. The level of 5-methyl-deoxycytosine increased from 3.8 to 7.8%, indicating possible interference of this marine toxin with DNA regulation, replication, and expression. Higher concentrations of OA inhibited DNA synthesis, but failed to increase the rate of DNA methylation.
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