✦ LIBER ✦
5-methoxytryptamine in rat hypothalamus and human CSF. A fact or artifact?
✍ Scribed by N. Narasimhachari; E. Kempster; M. Anbar
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 425 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1076-5174
No coin nor oath required. For personal study only.
✦ Synopsis
Using thin-layer chromatography fluorometry, gas chromatography mass spectrometry and field ionization mass spectrometry the findings of Green et al. regarding the identification of 5-methoxytryptamine in rat hypothalamus could not be confirmed. On derivatization using pentafluoropropionic anhydride, melatonin underwent partial transacylation to yield 5-methoxytryptamine as one of the by-products. We thus consider the finding of 5-methoxytryptamine in rat hypothalamus by mass fragmentography as an artifact forming from melatonin, which is normally present in rat hypothalamus.