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5-Hydroxymethylcytosine-Mediated DNA Demethylation in Stem Cells and Development

✍ Scribed by Sun, Wenjia; Guan, Minxin; Li, Xuekun

Book ID: 121456119
Publisher: Mary Ann Liebert
Year: 2014
Tongue: English
Weight: 461 KB
Volume: 23
Category: Article
ISSN: 1547-3287
DOI: 10.1089/scd.2013.0428

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## Abstract Following gene targeting, a __loxP__‐neo‐__loxP__ cassette was introduced into ES cells. The presence of a selectable marker such as neo in the targeted allele may result in gene interference in flox mice or unexpected phenotypes due to genetic ambiguity in direct knockout mice. Typical