5-fluorouracil metabolite patterns in viable and necrotic tumor areas of murine colon carcinoma determined by 19F NMR spectroscopy
✍ Scribed by Yvonne J. L. Kamm; Arend Heerschap; Gert Rosenbusch; Ivonne M. C. M. Rietjens; Jacques Vervoort; D. J. Theo Wagener
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 548 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
High‐resolution ^19^F NMR spectroscopy at 9.4 T was used to study the difference in the metabolite pattern of 5‐fluorouracil (5‐FU) between viable and necrotic tissues of C38 murine colon tumors grown in C57BI/6 mice. Studies were performed on perchloric acid extracts of these tumor fractions after 5‐FU treatment. The ^19^F nuclear magnetic resonance spectra exhibited resonances representing 5‐FU, the catabolites α‐fluoro‐β‐ureidopropionic acid and α‐fluoro‐β‐alanine, as well as several fluoronucleotide anabolites. The absolute concentrations of anabolites and catabolites and the anabolite‐to‐catabolite ratio were significantly lower in the necrotic fraction than in the viable tumor fraction 50 min after administration of 5‐FU, whereas the absolute concentration of 5‐FU was the same. Therefore, in 5‐FU metabolism studies with NMR spectroscopy, it is important to consider the necrotic contribution to the tumor volume.