5-Azacytidine-induced 6-thioguanine resistance at the gpt locus in AS52 cells: Cellular response
✍ Scribed by Diane L. Spencer; William J. Caspary; Kimberly C. Hines; Kenneth R. Tindall
- Book ID
- 102655912
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 670 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Treatment of AS52 cells with 5azacytidine resulted in an induction of 6-thioguanine-resistant (6TG') colonies, which reached a maximum by an expression time of 9 days. Dose responses for both cytotoxicity and mutation induction were determined following treatment with Sazacytidine. At 20 pM treatment, 5-azacytidine exposure resulted in about 50% survival. Mutant frequency reached a maximum at 10 pM. At concentrations between 10 and 20 @A, Sazacytidine was a potent mutagen but did not exhibit a dose response. Although many compounds both induce cell death and affect the growth rate of cells, Sazocytidine specifically induced cell death and did not affect the doubling time of the surviving treated cell population.