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4517290 Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor

Book ID
122735156
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
67 KB
Volume
3
Category
Article
ISSN
0734-9750

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Comparison of two methods of preparing e
Comparison of two methods of preparing enzyme-antibody conjugates: Application of these conjugates for enzyme immunoassay
โœ Michael J. O'Sullivan; Ernesto Gnemmi; David Morris; Giorgio Chieregatti; Alan D ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 783 KB

Two methods of preparing enzyme-antibody conjugates were evaluated. High yields of conjugate were obtained with both methods. The first procedure utilizes the homobifunctional crosslinking reagent N,N'-o-phenylenedimaleimide. Sulfydryl residues were introduced into second antibodies by reaction wit