✦ LIBER ✦
4′,5′-dimethoxy-6-carboxyfluorescein: A novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays
✍ Scribed by Pyare L. Khanna; Edwin F. Ullman
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 416 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A new nonfluorescent dye, 4',5'-dimethoxy-6-carboxyfluorescein has been prepared which is a useful energy acceptor for energy transfer measurements from fluorescein in the study of distances within macromolecular assemblies. The R,, value of 62 A for the new dye-fluorescein pair is exceptionally high. The utility of this dye pair has been demonstrated in a fluorescence excitation transfer immunoassay for morphine.