4-Methylmorpholine N-oxide-methyl sulfoxide soluble glucan of Piptoporus betulinus

✍ Scribed by Paul G. James; Robert Cherniak

• Book ID: 102993645
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 410 KB
• Volume: 206
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(90)84015-m

✦ Synopsis

Fungal and yeast cell walls contain a large proportion of (1+3)-p-and (1+6)-j?-D-glucopyranans . lm3 Recently (1 +3)-cl-D-glucopyranans were identified as common yeast cell-wall components &. These glucans function as virulence factors7, storage polysaccharides', and contributors to the structural integrity of the cell wall. 13C-N.m.r. spectra of water-insoluble glucans from Streptococcus mutans, that contain both (1+3)-CI-and (1+6)+linkages have been acquired from native polysaccharides and from a dextranase-degraded derivative'*". These spectra were noted for their broad linewidths due, in part, to incomplete digestion of (1+6)+linked residues. Linear (l-+3)-linked residues and branched (l-+3) residues were not distinguishable in these spectra.

The insolubility of these D-glucans in water makes hydrolysis and analysis difficult and extraction of water-insoluble cell-wall components with alkaline reagents can also cause degradation of the polysaccharides. The solvents 4-methylmorpholine N-oxide (CMMNO) and methyl sulfoxide (Me,SO) are capable of complete solubilization of plant cell walls when used in tandem".12. 4-MMNO, for example, is an excellent solvent for polysaccharides I3914 The utility of these reagents in the isolation .