4-Hydroxynonenal interacts with tubulin by reacting with its functional -SH groups

✍ Scribed by Antonella Olivero; Antonella Miglietta; Elena Gadoni; Ludovica Gabriel

• Book ID: 102789306
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 561 KB
• Volume: 8
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.290080204

✦ Synopsis

4-Hydroxynonenal, which is one of the most important products of lipid peroxidation, alters microtubular organization and structure in 3T3 fibroblasts. Changes in cell shape and the disappearance of microtubules are observed by immunofluorescence after incubation with the aldehyde, and the colchicine binding activity of tubulin from 3T3 cells is modified. Moreover, the aldehyde determines a decrease in the ability of purified tubuiin to polymerize and to bind colchicine. These effects may be related to the interaction of the aldehyde with functional -SH groups of tubulin which are necessary for protein integrity and functions. Indeed, the addition of cysteine protects against the damaging effects of the aldehyde.