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4-Hydroxynonenal induces membrane perturbations and inhibition of basal prostacyclin production in endothelial cells, and migration of monocytes.

✍ Scribed by Nicanor I. Moldovan; Florea Lupu; Leni Moldovan; Nicolae Simionescu

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
655 KB
Volume
18
Category
Article
ISSN
1065-6995

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✦ Synopsis

ABSTRACT

Cultured bovine aortic endothelial cells (BAEC) were incubated for 5 days with 10^−5^ 4‐hydroxynonenal (HN). HN treated BAEC and controls were either (i) further incubated with ^125^I‐polymyxin B (IPxB) or with radioiodinated, inactivated coagulation factor Xa (IFXai) as markers of membrane phospholipid perturbation, or (ii) assayed for the synthesis of prostacyclin (PGI~2~) and thromboxane A~2~ (TXA~2~). Rabbit blood mononuclear cells enriched in monocytes (MC) were isolated and assayed for chemotactic response to HN. The results showed six ‐ fold increases of IPxB and IFXai binding to BAEC treated with HN, as compared to untreated controls. We also found in HN treated cells a marked inhibition of PGI~2~ synthesis, but an unmodified TXA~2~ production. In addition, HN in the 10‐5‐10‐10 M range induced oriented migration of MC.

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