[3H]spiperone binding sites in rat primary glial cultures, C6 glioma, and B104 neuroblastoma

✍ Scribed by J. A. Severson; J. S. de Vellis; C. E. Finch

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 333 KB
• Volume: 9
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490090104

✦ Synopsis

Binding sites for [iH]spiperone were detected on membranes from primary glial cultures from neonatal rat cortex and striatum and from the C6 glioma cells.

['HISpiperone binding was displacable by d-butaclamol. However, competition studies suggest that ['Hlspiperone binding to primary glial cultures was mainly to serotonergic sites, and binding to the C6 glioma was alpha-adrenergic. No specific binding was detected to membranes from B 104 neuroblastoma cells. Although [?H]spiperone binding to glial sites on whole striatum under generally used conditions is small (about lo%), the striatal glial hyperactivity that is often associated with neuronal degeneration could lead to an overestimation of presumed neuronal binding sites for dopaminergic ligands.