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3H-Thymidininkorporation nach Isoleucinlimitation in “stringent” und “relaxed” kontrollierten Stämmen von Escherichia coli

✍ Scribed by Dr. M. Hecker; A. Schroeter

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 412 KB
Volume: 24
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.19840240111

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✦ Synopsis

Stringent and relaxed strains of E . coli subjected to isoleucine starvation were examined by followwing the incorporation of 3H-thymidine into chromosomal DNA. After valine treatment to trigger an isoleucine deprivation (p)ppGpp is synthesized in the stringent strain only. Remarkable differences in the morphology of the amino acid starved cells of the stringent and relaxed strains can be observed. Upon isoleucine limitation 3H-tliymidine incorporation into DNA is reduced in both strains, but this inhibition is remarkably delayed in the relaxed strain. Our result show that the reduction of rhromosomal DNA synthesis during amino acid limitation ocrurs also without ppGpp, but in the presence of ppGpp this process is accelerated.

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