3H-muscimol binding in synaptosomal fractions from bovine and developing rabbit retinas

✍ Scribed by Dr. Dianna A. Redburn; Cheryl K. Mitchell

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 536 KB
• Volume: 6
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490060406

✦ Synopsis

Abstract

Bovine and rabbit retina synaptosomal fractions bind the potent and specific GABA agonist, [^3^H]‐muscimol with high affinity and limited capacity. The high degree of pharmacological specificity and the subcellular distribution of these binidng sites are similar to those reported for [^3^H]‐GABA binding sites. These observations suggest that these sites represent the recongition sites for GABA receptors. The specific binding of [^3^H]‐muscimol in retinal homogenates from different aged rabbits reveal a distinct developmental profile with a fivefold to six fold increase in binding between days 5 and 13. Thus, it appears that GABA receptor development continues after eye opening in rabbits (day 9–10) and that receptor maturation is delayed by at least two to three days with respect to published reports of GABA uptake and evoked release.