3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo
✍ Scribed by H. Watanabe; Y. Ishihara; K. Okamoto; K. Oshio; T. Kanamatsu; Y. Tsukada
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 349 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
A method for spatially three-dimensional (3D) localized twodimensional (2D) 1 H-13 C correlation spectroscopy, localized HSQC, is proposed. This method has the following special feature in the preparation period. The 180°( 13 C) and 180°( 1 H) pulses are separated in time, and the 180°( 13 C) pulse is applied at 1/(4 1 J CH ) before the 90°( 1 H) polarization transfer pulse. The preparation (echo) period 2 can then be set substantially longer than 1/(2 1 J CH ), so that even in a whole-body system, slice-selective 90°( 1 H) pulses and gradient pulses can be applied in that period. The localization capabilities of this method were confirmed in a phantom experiment. The 3D localized 2D 1 H-13 C correlation spectra from a monkey brain in vivo were obtained after [1-13 C]glucose injection, and amino acid metabolism was detected; that is, [4-13 C]glutamate appeared immediately after the injection, followed by the appearance of [2-13 C]glutamate, [3-13 C]glutamate, and [4-13 C]glutamine. Magn Reson Med 43: 200 -210, 2000.