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3d Hcch-Cosy-Tocsy experiment for the assignment of ribose and amino acid side chains in 13C labeled RNA and protein

✍ Scribed by Weidong Hu; Lazaros T. Kakalis; Licong Jiang; Feng Jiang; Xiaomei Ye; Ananya Majumdar

Book ID
110262960
Publisher
Springer Netherlands
Year
1998
Tongue
English
Weight
110 KB
Volume
12
Category
Article
ISSN
0925-2738

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We have prepared RNAs uniformly 13C-labeled in the ribose ring and with the H3',H4',H5'/H5" protons specifically replaced with deuterons (3',4',5'/5"-2H-13C, termed d4-13C ribose), which offers the advantage of spectral simplification without sacrifice of sensitivity for the remaining protons. A 3D