3D Accordion Spectroscopy for Measuring15N and13CO Relaxation Rates in Poorly Resolved NMR Spectra

✍ Scribed by Peter A. Carr; David A. Fearing; Arthur G. Palmer III

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 204 KB
• Volume: 132
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1998.1374

✦ Synopsis

An experimental approach for the measurement of nuclear magnetic spin relaxation rate constants that combines triple-resonance techniques and accordion spectroscopy is described. Pulse sequences are discussed for the measurement of backbone 15N and 13CO R1 relaxation rate constants. The three-dimensional HNCO triple-resonance technique is employed to gain improved spectral resolution over conventional two-dimensional methods by frequency labeling both the 15N and 13CO spins. Accordion spectroscopy is used to reduce the dimensionality of the relaxation experiment. The "negative-time accordion" approach (A. M. Mandel and A. G. Palmer (1994), J. Magn. Reson. A 110, 62-72) is used for extracting rate constants from the t1 interferograms. The experiments are demonstrated using a 13C/15N isotopically enriched sample of the third fibronectin type III domain of human tenascin.