34S/32S fractionation by sulfate-reducing microbial communities in estuarine sediments
✍ Scribed by Marjolijn C. Stam; Paul R.D. Mason; Anniet M. Laverman; Céline Pallud; Philippe Van Cappellen
- Book ID
- 116504497
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 537 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0016-7037
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In this study, we describe a competitive polymerase chain reaction (PCR) for the quantification of the sequences of dsrAB in sulfate-reducing microorganisms. We used the dsr1F/dsr4R set of primers, previously designed by Wagner et al. (1998), and a competitor sequence was constructed from the dsrAB
## Abstract Molecular investigations of the sulfate reducing bacteria that target the dissimilatory sulfite‐reductase subunit A gene (__dsr__ A) are plagued by the nonspecific performance of conventional PCR primers. Here we describe the incorporation of the FailSafe™ PCR System to optimize environ