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327 POSTER Improved method for preparation of formalin-fixed paraffin-embedded tissue for mass spectrometric analysis

✍ Scribed by J. Cole; R. Streeper; D. Campos; E. Izbicka

Book ID
118623955
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
203 KB
Volume
4
Category
Article
ISSN
1359-6349

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A modification of the Hedley-method for flow cytometric DNA analysis of paraffin-embedded tissues is presented. Dewaxed and dehydrated tissue from paraffin blocks was incubated with subtilisin Carlsberg (pronase, Sigma protease XXIV) and then stained directly without washing and centrifugation. The