A recently described alternative method for determining free [Mg 2 ] in cells and tissue with 31 P NMR spectroscopy, which is based on the peak-height ratio of the b and a resonances of ATP (h b/a ) [J. Biol. Chem. 271, 21141-21150 (1996)], was found to have numerous problems. These problems include
31P NMR lineshapes of β-P (ATP) in the presence of mg2+ and ca2+ : estimate of exchange rates
✍ Scribed by K.V. Vasavada; B.D. Ray; B.D. Nageswara Rao
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 762 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0162-0134
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Recently, Clarke et al. (Clarke K, Kashiwaya Y, King MT, Gates D, Keon CA, Cross HR, Radda GK, Veech RL. The b/a peak height ratio of ATP. A measure of free [Mg 2 + free ] using 31 P NMR, J. Biol. Chem. 1996;271:21142-21150.) reported a new method to noninvasively determine the concentration of intr
## Abstract Previous investigators found that cyanide (CN^−^) is a potent inhibitor of renal Na^+^ transport, while the uncoupling agent 2,4‐dinitrophenol (DNP) and fructose (both which lower ATP levels) are weak transport inhibitors. To examine the disparate effects of these substances measurement
## Abstract The ability to measure ATP synthesis rates using ^31^P‐NMR spectroscopy is demonstrated in the normal, ischemic, and postischemic myocardium __in vivo.__ Cardiopulmonary bypass (CBP) was employed to induce 20 min of global myocardial ischemia, and to conduct magnetization transfer measu