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31P NMR detection of mobile dog brain phospholipids

✍ Scribed by S. Cerdan; V. Harihara Subramanian; M. Hilberman; J. Cone; J. Egan; B. Chance; J. R. Williamson

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
395 KB
Volume
3
Category
Article
ISSN
0740-3194

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✦ Synopsis

The in vivo dog brain "P NMR spectrum has a large peak in the phosphodiester region accounting for more than 35% of the total observable phosphorus metabolites. It is possible to reduce the intensity of this peak by off-resonance saturation. To characterize the nature of this peak, extracts of dog brain frozen in situ were analyzed by high resolution "P NMR. ATP, phosphocreatine, inorganic phosphate, and phosphomonoesters were recovered in appropriate amounts in the methano1:HCI extract. However, acid soluble phosphodiesters accounted for only 8% of the observable phosphorus. More NMR observable phosphodiesters were selectively recovered following CHC1,:methanol extraction of phospholipids.

These results suggest that the in vivo phosphodiester resonance has substantial contributions from a fraction of mobile brain phospholipids. o 1986 Academic h, Inc.

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