31P NMR and enzymatic analysis of cytosolic phosphocreatine, ATP, Pi and intracellular pH in the isolated working perfused rat heart
β Scribed by Geoffrey P. Dobson; Richard L. Veech; Janet V. Passonneau; Kumpai Kobayashi; Toshiro Inubushi; Susanna Wehrli; Shoko Nioka; Britton Chance
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 1002 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0952-3480
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## Abstract To quantify metabolite and cation concentrations using NMR spectroscopy, the volumes of intracellular and extracellular spaces must be known. We describe a simple ^31^P NMR spectroscopic method that employs dimethyl methylphosphonate (DMMP) as a marker of total water space and phenylpho
## Abstract The triple quantum filtered ^23^Na NMR method is applied here to measure the effects of EIPA, a specific inhibitor of the Na^+^/H^+^antiporter, on relative intracellular sodium concentrations in isolated working hearts at baseline, during ischemia, and at subsequent reperfusion. In anal