31P-NMR analysis of lethal and sublethal lesions produced by KCl-intoxication in the zebra mussel,Dreissena polymorpha
✍ Scribed by O'Donnell, J. Michael; Durand, Marite E.; Robitaille, Pierre-Marie L.; Fisher, Susan Warwick; Stromberg, Paul C.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 880 KB
- Volume
- 276
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Noninvasive phosphorus-31 nuclear magnetic resonance spectroscopy (31P-NMR) was utilized to investigate the mechanism of potassium chloride (KC1) toxicity in zebra mussels. 31P-NMR spectra showed several bioenergetically important high energy phosphates, including ATP, ADP, phosphoarginine, and inorganic phosphate. Exposure of mussels to 8.6 mM KC1 caused changes in these metabolites and proved lethal to the mussels when exposed over a 24 h period. This result matched that of the molluscicide Bayluscide. Here, losses in phosphoarginine and ATP were accompanied by a rise in inorganic phosphate and a shift toward intracellular acidosis. KC1 levels below 8.6 mM caused marked changes in mussel high energy phosphates and pHi. However,