31P MAS-NMR of human erythrocytes: Independence of cell volume from angular velocity

✍ Scribed by P.W. Kuchel; W.A. Bubb; S. Ramadan; B.E. Chapman; D.J. Philp; M. Coen; J.E. Gready; P.J. Harvey; A.J. McLean; J. Hook

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 308 KB
• Volume: 52
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.20139

✦ Synopsis

Abstract

^31^P magic angle spinning NMR (MAS‐NMR) spectra were obtained from suspensions of human red blood cells (RBCs) that contained the cell‐volume‐sensitive probe molecule, dimethyl methylphosphonate (DMMP). A mathematical representation of the spectral‐peak shape, including the separation and width‐at‐half‐height in the ^31^P NMR spectra, as a function of rotor speed, enabled us to explore the extent to which a change in cell volume would be reflected in the spectra if it occurred. We concluded that a fractional volume change in excess of 3% would have been detected by our experiments. Thus, the experiments indicated that the mean cell volume did not change by this amount even at the highest spinning rate of 7 kHz. The mean cell volume and intracellular ^31^P line‐width were independent of the packing density of the cells and of the initial cell volume. The relationship of these conclusions to other non‐NMR studies of pressure effects on cells is noted. Magn Reson Med 52:663–668, 2004. © 2004 Wiley‐Liss, Inc.