3-Hydroxypropylmercapturic acid: A biologic marker of exposure to allylic and related compounds

✍ Scribed by Radhika Sanduja; G. A. S. Ansari; Paul J. Boor

• Book ID: 102293941
• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 332 KB
• Volume: 9
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.2550090406

✦ Synopsis

3-Hydroxypropylmercapturic acid [bOHPrMCA, S-(3-hydroxypropyl)-N-acetyl-~-cysteine] was quantitatively measured by high-performance liquid chromatography (HPLC) in the urine of rats given allylamine.HCI (5, 25, 50, 100 and 150 mg kg-I), acrolein (13 mg kg,'), allylalcohol (64 mg kg-I), allylchloride (76 mg kg-I), allylbromide (120 mg kg-'), allylcyanide (115 mg) and cyclophosphamide (160 mg kg-') by gavage in water.

3-OHPrMCA was measured by HPLC in 24-h urine collections; the lower detection limit was 1.25 p.g or 5.6 nmol ml-'. Various doses of allylamine resulted in 3-OHPrMCA excretion at a fairly constant percentage of the dose, ca. 4 4 4 8 % at 0-24 h and 3% at 24-48 h, indicating rapid metabolism through glutathione conjugation in the first 24h. Similarly, 3-OHPrMCA was recovered in the urine of rats given acrolein (78.5%), allylalcohol (28.3%), allylchloride (21.5%), allylbromide (3.0%), allylcyanide (3.7%) and cyclophosphamide (2.6%). These data indicate that 3-OHPrMCA can be used as a marker of exposure to allylic and other compounds that lead to the metabolic formation of acrolein.