3-Hydroxy-3-methylglutaryl coenzyme a reductase from the latex of Hevea brasiliensis
β Scribed by Rapepun Wititsuwannakul; Dhirayos Wititsuwannakul; Plueng Suwanmanee
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 332 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0031-9422
No coin nor oath required. For personal study only.
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A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2
## Abstract Elevated 3βhydroxyβ3βmethylglutaryl coenzyme A (HMGβCoA) reductase expression supports synthesis of prenyl pyrophosphate intermediates required for tumor growth. In this study, the copy number of HMGβCoA reductase mRNA was determined in solid tumor and leukemic cell lines using competit