2D or not 2D

✍ Scribed by Stephen J Fey; Peter Mose Larsen

• Book ID: 104414495
• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 186 KB
• Volume: 5
• Category: Article
• ISSN: 1367-5931
• DOI: 10.1016/s1367-5931(00)00167-8

✦ Synopsis

2D gel electrophoresis is the technology that everyone loves to hate-it requires manual dexterity and precision to reproduce precisely and is thus not well-suited as a high-throughput technology. Although almost everyone would like to replace it, the resolution and sensitivity it offers are exquisite and unsurpassed if one wants a global view of cellular activity. There have been several recent developments, for example, the detection of low abundance proteins, and the resolution possible with narrow-range IPG gels.