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288 Whole genome deep sequencing decomposes the genome evolution of renal cell carcinomas

✍ Scribed by Wu, S.; Mei, H.


Book ID
122561916
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
107 KB
Volume
13
Category
Article
ISSN
1569-9056

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We applied a differential cloning procedure, the in-gel competitive reassociation (IGCR) method, to clone altered genomic sites from the whole genomes of renal cell carcinoma cells. After four rounds of IGCR, we obtained from two patients libraries enriched 1000-and 2500-fold for differential DNA fr